Detection method
\nColorimetric
\nPrecision
\nIntra-assay
\nSample n Mean SD CV%
\nOverall < 10%\nInter-assay\nSample n Mean SD CV%\nOverall < 12%\nSample type\nCell culture extracts, Tissue Extracts\nAssay type\nSandwich (quantitative)\nSensitivity\n< 80 pg\/ml\nRange\n68.59 pg\/ml - 50000 pg\/ml\nRecovery\n116 %\n\nSample specific recovery\nSample type Average % Range\nCell culture extracts 96.22 88% - 105%\nTissue Extracts 136.5 126% - 145%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Rat\nProduct overview\nAbcam\u2019s IL-1 beta Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in cell lysates and tissue lysates.\n\n\nThis assay employs an antibody specific for rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.\n\nNotes\nAbcam has not and does not intend to apply for the REACH Authorisation of customers\u2019 uses of products that contain European Authorisation list (Annex XIV) substances.\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 80 pg\/ml
\nRange: 68.59 pg\/ml – 50000 pg\/ml
\nSample type: Cell culture extracts, Tissue Extracts
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Rat<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27392"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27392"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27392"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}