Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 30 pg\/ml\nRange\n40.96 pg\/ml - 10000 pg\/ml\nRecovery\n119 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 130.2 118% - 138%\nPlasma 122.8 109% - 138%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Rat\nProduct overview\nAbcam\u2019s IL-6 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat IL-6 in plasma or cell culture supernatants.\n\n\nThis assay employs an antibody specific for Rat IL-6 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\n\nDue to the relatively low abundance of IL-6 in normal rat plasma, this kit may not be suitable for use with this sample type. We have not been able to detect endogenous Rat IL-6 in normal serum with ab100772, only in serum spiked with Rat IL-6.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 30 pg\/ml
\nRange: 40.96 pg\/ml – 10000 pg\/ml
\nSample type: Cell culture supernatant, Plasma
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Rat<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27396"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27396"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27396"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}