Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Serum, Plasma
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 50 pg\/ml\nRange\n0.05 ng\/ml - 40 ng\/ml\nRecovery\n100 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 95.42 83% - 108%\nSerum 109.78 99% - 118%\nPlasma 107.6 98% - 118%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Rat\nProduct overview\nAbcam\u2019s MMP8 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP8 in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.\n\nThis assay employs an antibody specific for MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 50 pg\/ml
\nRange: 0.05 ng\/ml – 40 ng\/ml
\nSample type: Cell culture supernatant, Plasma, Serum
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Rat<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27403"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27403"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27403"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}