Detection method
\nColorimetric
\nSample type
\nCell culture supernatant, Cell culture extracts, Tissue Extracts
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n< 25 pg\/ml\nRange\n82.3 pg\/ml - 20000 pg\/ml\nRecovery\n92 %\n\nSample specific recovery\nSample type Average % Range\nCell culture extracts 93.17 81% - 105%\nTissue Extracts 92.48 80% - 104%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Rat\nProduct overview\nAbcam\u2019s TNF alpha ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in cell lysates and tissue lysates.\n\nThis assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nNotes\nAbcam has not and does not intend to apply for the REACH Authorisation of customers\u2019 uses of products that contain European Authorisation list (Annex XIV) substances.\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 25 pg\/ml
\nRange: 82.3 pg\/ml – 20000 pg\/ml
\nSample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Rat<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27408"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27408"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27408"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}