{"id":27417,"date":"2021-02-14T18:30:16","date_gmt":"2021-02-14T15:30:16","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-myoglobin-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:36:24","modified_gmt":"2021-02-14T20:36:24","slug":"human-myoglobin-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-myoglobin-elisa-kit-96-tests\/","title":{"rendered":"Human Myoglobin ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nSerum<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 5 ng\/ml<br \/>\nRange<br \/>\n5 ng\/ml &#8211; 1000 ng\/ml<br \/>\nRecovery<br \/>\n102.8 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nSerum 102.8 39ng\/ml &#8211; 780ng\/ml<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Myoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myoglobin in Human serum.<\/p>\n<p>A 96-well plate has been precoated with anti-Myoglobin antibodies. Samples and standards and are added to the wells, where Myoglobin in the sample and standards binds to the precoated antibody. Added anti-Myoglobin HRP conjugate binds to this antibody-Myoglobin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Myoglobin in the sample and the intensity is measured at 450 nm.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 5 ng\/ml<br \/>\nRange: 5 ng\/ml &#8211; 1000 ng\/ml<br \/>\nSample type: Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27417"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27417"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27417"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}