{"id":27422,"date":"2021-02-14T18:30:19","date_gmt":"2021-02-14T15:30:19","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-triiodothyronine-elisa-kit-free-total-t3-96-tests\/"},"modified":"2021-02-14T23:36:32","modified_gmt":"2021-02-14T20:36:32","slug":"human-triiodothyronine-elisa-kit-free-total-t3-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-triiodothyronine-elisa-kit-free-total-t3-96-tests\/","title":{"rendered":"Human Triiodothyronine ELISA Kit (free + total T3)- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nSerum<br \/>\nAssay type<br \/>\nCompetitive<br \/>\nSensitivity<br \/>\n= 0.2 ng\/ml<br \/>\nRange<br \/>\n0.2 ng\/ml &#8211; 10 ng\/ml<br \/>\nRecovery<br \/>\n106 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nSerum 106 0.68ng\/ml &#8211; 5.81ng\/ml<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.<\/p>\n<p>A 96-well plate has been pre-coated with goat anti-mouse IgG. Samples, standards and the mouse anti-Triiodothyronine Reagent and Triiodothyronine-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.<\/p>\n<p>This kit detects total and free T3. To detect only free T3 please use ab108663.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.2 ng\/ml<br \/>\nRange: 0.2 ng\/ml &#8211; 10 ng\/ml<br \/>\nSample type: Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Competitive<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27422"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27422"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}