{"id":27425,"date":"2021-02-14T18:30:21","date_gmt":"2021-02-14T15:30:21","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/17-beta-estradiol-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:36:34","modified_gmt":"2021-02-14T20:36:34","slug":"17-beta-estradiol-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/17-beta-estradiol-elisa-kit-96-tests\/","title":{"rendered":"17 beta Estradiol ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nUrine, Serum, Hep Plasma, EDTA Plasma, Cit plasma<br \/>\nAssay type<br \/>\nCompetitive<br \/>\nSensitivity<br \/>\n8.68 pg\/ml<br \/>\nRange<br \/>\n20 pg\/ml &#8211; 2000 pg\/ml<br \/>\nRecovery<br \/>\n101.09 %<\/p>\n<p>Assay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Mouse, Rat, Human<br \/>\nPredicted to work with: Mammals<br \/>\nProduct overview<br \/>\nAbcam\u2019s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).<\/p>\n<p>A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 8.68 pg\/ml<br \/>\nRange: 20 pg\/ml &#8211; 2000 pg\/ml<br \/>\nSample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Competitive<br \/>\nReacts with: Mouse, Rat, Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27425"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27425"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}