{"id":27434,"date":"2021-02-14T18:30:26","date_gmt":"2021-02-14T15:30:26","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-prolactin-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:36:42","modified_gmt":"2021-02-14T20:36:42","slug":"human-prolactin-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-prolactin-elisa-kit-96-tests\/","title":{"rendered":"Human Prolactin ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nSerum<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n0.25 ng\/ml<br \/>\nRange<br \/>\n5 ng\/ml &#8211; 100 ng\/ml<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.<\/p>\n<p>Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.<\/p>\n<p>Get results in 90 minutes with Human Prolactin ELISA Kit (ab189570) from our SimpleStep ELISA\u00ae range.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.25 ng\/ml<br \/>\nRange: 5 ng\/ml &#8211; 100 ng\/ml<br \/>\nSample type: Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27434"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27434"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27434"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}