Sample type
\nCell culture supernatant, Urine
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n= 0.78 ng\/ml
\nRange
\n1.563 ng\/ml – 100 ng\/ml
\nRecovery
\n98 %<\/p>\n
Assay time
\n4h 00m
\nAssay duration
\nMultiple steps standard assay
\nSpecies reactivity
\nReacts with: Pig
\nProduct overview
\nAbcam\u2019s Albumin Pig in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of pig Albumin levels in urine and cell culture supernatants.<\/p>\n
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.<\/p>\n
The entire kit may be stored at -20\u00b0C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.<\/p>\n
Platform
\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 0.78 ng\/ml
\nRange: 1.563 ng\/ml – 100 ng\/ml
\nSample type: Cell culture supernatant, Urine
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Pig<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27520"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27520"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27520"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}