{"id":27524,"date":"2021-02-14T18:31:51","date_gmt":"2021-02-14T15:31:51","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-alpha-1-antitrypsin-elisa-kit-serpina1-96-tests-2\/"},"modified":"2021-02-14T23:38:11","modified_gmt":"2021-02-14T20:38:11","slug":"human-alpha-1-antitrypsin-elisa-kit-serpina1-96-tests-2","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-alpha-1-antitrypsin-elisa-kit-serpina1-96-tests-2\/","title":{"rendered":"Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture media, Cerebral Spinal Fluid<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 0.32 ng\/ml<br \/>\nRange<br \/>\n0.391 ng\/ml &#8211; 25 ng\/ml<br \/>\nRecovery<br \/>\n103 %<\/p>\n<p>Assay time<br \/>\n4h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nHuman alpha 1 Antitrypsin  ELISA kit (SERPINA1) (ab108799) is a sandwich ELISA kit for the quantitative measurement of alpha 1 Antitrypsin in urine, milk, saliva, CSF, cell lysate and cell culture supernatants.<\/p>\n<p>An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.<\/p>\n<p>Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA\u00ae range.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.32 ng\/ml<br \/>\nRange: 0.391 ng\/ml &#8211; 25 ng\/ml<br \/>\nSample type: Cell culture media, Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27524"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27524"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}