{"id":27527,"date":"2021-02-14T18:31:53","date_gmt":"2021-02-14T15:31:53","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-apolipoprotein-ai-elisa-kit-apoa1-96-tests\/"},"modified":"2021-02-14T23:38:13","modified_gmt":"2021-02-14T20:38:13","slug":"human-apolipoprotein-ai-elisa-kit-apoa1-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-apolipoprotein-ai-elisa-kit-apoa1-96-tests\/","title":{"rendered":"Human Apolipoprotein AI ELISA Kit (APOA1)- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cerebral Spinal Fluid<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 3 ng\/ml<br \/>\nRange<br \/>\n6.25 ng\/ml &#8211; 100 ng\/ml<br \/>\nRecovery<br \/>\n97 %<\/p>\n<p>Assay time<br \/>\n4h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in plasma, serum, urine, saliva, milk, CSF and cell culture samples.<\/p>\n<p>An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.<\/p>\n<p>Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA\u00ae range.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 3 ng\/ml<br \/>\nRange: 6.25 ng\/ml &#8211; 100 ng\/ml<br \/>\nSample type: Cell culture extracts, Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27527"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27527"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27527"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}