{"id":27528,"date":"2021-02-14T18:31:54","date_gmt":"2021-02-14T15:31:54","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-apolipoprotein-ai-elisa-kit-apoa1-96-tests-2\/"},"modified":"2021-02-14T23:38:14","modified_gmt":"2021-02-14T20:38:14","slug":"human-apolipoprotein-ai-elisa-kit-apoa1-96-tests-2","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-apolipoprotein-ai-elisa-kit-apoa1-96-tests-2\/","title":{"rendered":"Human Apolipoprotein AI ELISA Kit (APOA1)- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nSerum, Plasma<br \/>\nAssay type<br \/>\nCompetitive<br \/>\nSensitivity<br \/>\n0.23 \u00b5g\/ml<br \/>\nRange<br \/>\n0.313 \u00b5g\/ml &#8211; 10 \u00b5g\/ml<br \/>\nRecovery<br \/>\n98 %<\/p>\n<p>Assay time<br \/>\n3h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nApolipoprotein AI Human (APOA1) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI levels in plasma and serum.<\/p>\n<p>An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Apolipoprotein AI is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Apolipoprotein AI captured in plate.<\/p>\n<p>Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA\u00ae range.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.23 \u00b5g\/ml<br \/>\nRange: 0.313 \u00b5g\/ml &#8211; 10 \u00b5g\/ml<br \/>\nSample type: Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Competitive<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27528"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27528"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27528"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}