{"id":27533,"date":"2021-02-14T18:31:57","date_gmt":"2021-02-14T15:31:57","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-apolipoprotein-h-elisa-kit-apoh-96-tests\/"},"modified":"2021-02-14T23:38:18","modified_gmt":"2021-02-14T20:38:18","slug":"human-apolipoprotein-h-elisa-kit-apoh-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-apolipoprotein-h-elisa-kit-apoh-96-tests\/","title":{"rendered":"Human Apolipoprotein H ELISA Kit (APOH)- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cell Lysate<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 0.45 ng\/ml<br \/>\nRange<br \/>\n0.625 ng\/ml &#8211; 40 ng\/ml<br \/>\nRecovery<br \/>\n98 %<\/p>\n<p>Assay time<br \/>\n4h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Apolipoprotein H (APOH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein H concentrations in Human plasma, serum, saliva, urine, milk and cell culture samples.<\/p>\n<p>An Apolipoprotein H specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein H specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein H captured in plate.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.45 ng\/ml<br \/>\nRange: 0.625 ng\/ml &#8211; 40 ng\/ml<br \/>\nSample type: Cell culture extracts, Cell culture supernatant, Cell Lysate, Milk, Plasma, Saliva, Serum, Urine<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27533"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27533"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}