{"id":27539,"date":"2021-02-14T18:34:51","date_gmt":"2021-02-14T15:34:51","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-complement-c3-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:38:23","modified_gmt":"2021-02-14T20:38:23","slug":"human-complement-c3-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-complement-c3-elisa-kit-96-tests\/","title":{"rendered":"Human Complement C3 ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nSaliva, Milk, Serum, Plasma<br \/>\nAssay type<br \/>\nCompetitive<br \/>\nSensitivity<br \/>\n= 0.2 \u00b5g\/ml<br \/>\nRange<br \/>\n0.117 \u00b5g\/ml &#8211; 7.5 \u00b5g\/ml<br \/>\nRecovery<br \/>\n99 %<\/p>\n<p>Assay time<br \/>\n3h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nHuman Complement C3 ELISA kit is a competitive immunoassay designed for the quantitative measurement of Complement C3 in human plasma and serum.<\/p>\n<p>A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection protein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C3 captured in plate.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.2 \u00b5g\/ml<br \/>\nRange: 0.117 \u00b5g\/ml &#8211; 7.5 \u00b5g\/ml<br \/>\nSample type: Milk, Plasma, Saliva, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Competitive<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27539"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27539"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27539"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}