Sample type
\nCell culture supernatant, Urine
\nAssay type
\nSandwich (quantitative)
\nSensitivity
\n= 12 ng\/ml
\nRange
\n12.5 ng\/ml – 800 ng\/ml
\nRecovery
\n97 %<\/p>\n
Assay time
\n4h 0m
\nAssay duration
\nMultiple steps standard assay
\nSpecies reactivity
\nReacts with: Dog
\nProduct overview
\nAbcam\u2019s Fibrinogen Canine in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of fibrinogen levels in urine and cell culture media.<\/p>\n
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.<\/p>\n
The entire kit may be stored at -20\u00b0C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.<\/p>\n
Platform
\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"
Key features and details
\nSensitivity: 12 ng\/ml
\nRange: 12.5 ng\/ml – 800 ng\/ml
\nSample type: Cell culture supernatant, Urine
\nDetection method: Colorimetric
\nAssay type: Sandwich (quantitative)
\nReacts with: Dog<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27553"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27553"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27553"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}