{"id":27576,"date":"2021-02-14T18:36:36","date_gmt":"2021-02-14T15:36:36","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-interferon-gamma-elisa-kit-ifng-96-tests-3\/"},"modified":"2021-02-14T23:39:02","modified_gmt":"2021-02-14T20:39:02","slug":"human-interferon-gamma-elisa-kit-ifng-96-tests-3","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-interferon-gamma-elisa-kit-ifng-96-tests-3\/","title":{"rendered":"Human Interferon gamma ELISA Kit (IFNG)- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n> 0.01 ng\/ml<br \/>\nRange<br \/>\n0.016 ng\/ml &#8211; 1 ng\/ml<br \/>\nRecovery<br \/>\n101 %<\/p>\n<p>Assay time<br \/>\n5h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Interferon gamma (IFNG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon gamma levels in cell culture supernatants, serum and plasma.<\/p>\n<p>An Interferon gamma specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Interferon gamma specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Interferon gamma captured in plate.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.01 ng\/ml<br \/>\nRange: 0.016 ng\/ml &#8211; 1 ng\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27576"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27576"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}