{"id":27587,"date":"2021-02-14T18:36:58","date_gmt":"2021-02-14T15:36:58","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-insulin-like-growth-factor-1-elisa-kit-igf1-96-tests\/"},"modified":"2021-02-14T23:39:14","modified_gmt":"2021-02-14T20:39:14","slug":"mouse-insulin-like-growth-factor-1-elisa-kit-igf1-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-insulin-like-growth-factor-1-elisa-kit-igf1-96-tests\/","title":{"rendered":"Mouse Insulin like Growth Factor 1 ELISA kit (IGF1)- 96 Tests"},"content":{"rendered":"<p>ample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 0.12 ng\/ml<br \/>\nRange<br \/>\n0.3 ng\/ml &#8211; 5 ng\/ml<br \/>\nRecovery<br \/>\n96 %<\/p>\n<p>Assay time<br \/>\n5h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Mouse<br \/>\nProduct overview<br \/>\nAbcam\u2019s Insulin like Growth Factor 1 (IGF1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 levels in plasma, serum and cell culture supernatants.<\/p>\n<p>An IGF1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IGF1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IGF1 captured in plate.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.12 ng\/ml<br \/>\nRange: 0.3 ng\/ml &#8211; 5 ng\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27587"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27587"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27587"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}