{"id":27613,"date":"2021-02-14T18:37:15","date_gmt":"2021-02-14T15:37:15","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-tissue-factor-pathway-inhibitor-elisa-kit-tfpi-96-tests\/"},"modified":"2021-02-14T23:39:40","modified_gmt":"2021-02-14T20:39:40","slug":"human-tissue-factor-pathway-inhibitor-elisa-kit-tfpi-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-tissue-factor-pathway-inhibitor-elisa-kit-tfpi-96-tests\/","title":{"rendered":"Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI)- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nCell culture supernatant, Milk, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n> 0.12 ng\/ml<br \/>\nRange<br \/>\n0.313 ng\/ml &#8211; 20 ng\/ml<br \/>\nRecovery<br \/>\n96.5 %<\/p>\n<p>Assay time<br \/>\n5h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.<\/p>\n<p>A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.<\/p>\n<p>Get results in 90 minutes with Human Tissue Factor Pathway Inhibitor ELISA Kit (ab188392) from our SimpleStep ELISA\u00ae range.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.12 ng\/ml<br \/>\nRange: 0.313 ng\/ml &#8211; 20 ng\/ml<br \/>\nSample type: Cell culture supernatant, Milk, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27613"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27613"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27613"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}