{"id":27614,"date":"2021-02-14T18:37:16","date_gmt":"2021-02-14T15:37:16","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-thrombin-antithrombin-complex-elisa-kit-tat-96-tests\/"},"modified":"2021-02-14T23:39:41","modified_gmt":"2021-02-14T20:39:41","slug":"human-thrombin-antithrombin-complex-elisa-kit-tat-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-thrombin-antithrombin-complex-elisa-kit-tat-96-tests\/","title":{"rendered":"Human Thrombin-Antithrombin Complex ELISA Kit (TAT)- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nCell culture supernatant, Milk, Serum, EDTA Plasma, Cit plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 0.84 ng\/ml<br \/>\nRange<br \/>\n0.84 ng\/ml &#8211; 120 ng\/ml<br \/>\nRecovery<br \/>\n95 %<\/p>\n<p>Assay time<br \/>\n4h 00m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nThrombin-Antithrombin Complex (TAT) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin-Antithrombin Complex concentrations in plasma, milk and cell culture supernatants.<\/p>\n<p>A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.<\/p>\n<p>Note: It is not recommended to perform spike recovery in plasma samples due to large amount of endogenous Anti-thrombin III (mg\/ml range) which will saturate the capture antibody and interfere with the binding of the TAT standard<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.84 ng\/ml<br \/>\nRange: 0.84 ng\/ml &#8211; 120 ng\/ml<br \/>\nSample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Milk, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27614"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27614"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}