{"id":27620,"date":"2021-02-14T18:37:30","date_gmt":"2021-02-14T15:37:30","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/rat-tnf-alpha-elisa-kit-96-tests-4\/"},"modified":"2021-02-14T23:39:46","modified_gmt":"2021-02-14T20:39:46","slug":"rat-tnf-alpha-elisa-kit-96-tests-4","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/rat-tnf-alpha-elisa-kit-96-tests-4\/","title":{"rendered":"Rat TNF alpha ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Sample type<br \/>\nCell culture supernatant<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n= 0.0082 ng\/ml<br \/>\nRange<br \/>\n0.016 ng\/ml &#8211; 1 ng\/ml<br \/>\nAssay time<br \/>\n4h 30m<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Rat<br \/>\nProduct overview<br \/>\nAbcam\u2019s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in rat cell culture supernatants.<\/p>\n<p>A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.<\/p>\n<p>The entire kit may be stored at -20\u00b0C for long term storage before reconstitution &#8211; Avoid repeated freeze-thaw cycles.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.0082 ng\/ml<br \/>\nRange: 0.0156 ng\/ml &#8211; 1 ng\/ml<br \/>\nSample type: Cell culture supernatant<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Rat<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27620"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27620"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27620"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}