{"id":27634,"date":"2021-02-14T18:37:39","date_gmt":"2021-02-14T15:37:39","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-tnfsf18-gitrl-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:39:58","modified_gmt":"2021-02-14T20:39:58","slug":"human-tnfsf18-gitrl-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-tnfsf18-gitrl-elisa-kit-96-tests\/","title":{"rendered":"Human TNFSF18 \/ GITRL ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 25 pg\/ml\nRange\n41 pg\/ml - 10000 pg\/ml\nRecovery\n97 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 87.83 67% - 100%\nSerum 116.9 108% - 123%\nPlasma 87.69 67% - 100%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Human\nProduct overview\nHuman TNFSF18 \/ GITRL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFSF18 \/ GITRL in serum, plasma and cell culture supernatants.\n\n\nThis assay employs an antibody specific for human TNFSF18 \/ GITRL coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFSF18 \/ GITRL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFSF18 \/ GITRL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFSF18 \/ GITRL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nNotes\nOptimization may be required with urine samples.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 25 pg\/ml<br \/>\nRange: 41 pg\/ml &#8211; 10000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27634"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27634"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27634"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}