{"id":27640,"date":"2021-02-14T18:37:43","date_gmt":"2021-02-14T15:37:43","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/human-entactin-elisa-kit-nid1-96-tests\/"},"modified":"2021-02-14T23:40:14","modified_gmt":"2021-02-14T20:40:14","slug":"human-entactin-elisa-kit-nid1-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/human-entactin-elisa-kit-nid1-96-tests\/","title":{"rendered":"Human Entactin ELISA Kit (NID1)- 96 Tests"},"content":{"rendered":"<p>Detection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 0.1 ng\/ml\nRange\n0.123 ng\/ml - 30 ng\/ml\nRecovery\n> 100 %<\/p>\n<p>Sample specific recovery<br \/>\nSample type Average % Range<br \/>\nCell culture supernatant 125.7 111% &#8211; 138%<br \/>\nSerum 132.3 123% &#8211; 145%<br \/>\nPlasma 131.4 122% &#8211; 147%<br \/>\nAssay duration<br \/>\nMultiple steps standard assay<br \/>\nSpecies reactivity<br \/>\nReacts with: Human<br \/>\nProduct overview<br \/>\nAbcam\u2019s Entactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Entactin in serum, plasma, cell culture supernatants.<\/p>\n<p>This assay employs an antibody specific for Human Entactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Entactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Entactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Entactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<\/p>\n<p>Notes<br \/>\nOptimization may be required with urine samples.<\/p>\n<p>Platform<br \/>\nMicroplate<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 0.1 ng\/ml<br \/>\nRange: 0.123 ng\/ml &#8211; 30 ng\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Human<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27640"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27640"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27640"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}