{"id":27644,"date":"2021-02-14T18:37:46","date_gmt":"2021-02-14T15:37:46","guid":{"rendered":"https:\/\/bgenz.com\/Bio\/product\/mouse-flt-3-ligand-elisa-kit-96-tests\/"},"modified":"2021-02-14T23:40:20","modified_gmt":"2021-02-14T20:40:20","slug":"mouse-flt-3-ligand-elisa-kit-96-tests","status":"publish","type":"product","link":"https:\/\/bgenz.com\/Bio\/product\/mouse-flt-3-ligand-elisa-kit-96-tests\/","title":{"rendered":"Mouse Flt 3 Ligand ELISA Kit- 96 Tests"},"content":{"rendered":"<p>Product name<br \/>\nMouse Flt 3 Ligand ELISA Kit<br \/>\nDetection method<br \/>\nColorimetric<br \/>\nSample type<br \/>\nCell culture supernatant, Serum, Plasma<br \/>\nAssay type<br \/>\nSandwich (quantitative)<br \/>\nSensitivity<br \/>\n< 15 pg\/ml\nRange\n20 pg\/ml - 5000 pg\/ml\nRecovery\n87 %\n\nSample specific recovery\nSample type Average % Range\nCell culture supernatant 90.49 67% - 103%\nSerum 91.81 83% - 99%\nPlasma 77.99 69% - 85%\nAssay duration\nMultiple steps standard assay\nSpecies reactivity\nReacts with: Mouse\nProduct overview\nAbcam\u2019s Flt3 Ligand Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Flt3 Ligand in serum, plasma and cell culture supernatants.\n\nThis assay employs an antibody specific for mouse Flt3 Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Flt3 Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\n\nPlatform\nMicroplate\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key features and details<br \/>\nSensitivity: 15 pg\/ml<br \/>\nRange: 20 pg\/ml &#8211; 5000 pg\/ml<br \/>\nSample type: Cell culture supernatant, Plasma, Serum<br \/>\nDetection method: Colorimetric<br \/>\nAssay type: Sandwich (quantitative)<br \/>\nReacts with: Mouse<\/p>\n","protected":false},"featured_media":17768,"comment_status":"open","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product\/27644"}],"collection":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/comments?post=27644"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media\/17768"}],"wp:attachment":[{"href":"https:\/\/bgenz.com\/Bio\/wp-json\/wp\/v2\/media?parent=27644"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}