17 OH Progesterone ELISA Kit- 96 Tests
Available on backorder
Expiry Date
Key features and details
Sensitivity: 0.05 ng/ml
Sample type: Cit plasma, Hep Plasma, Serum
Detection method: Colorimetric
Assay type: Competitive
EGP 19,152.00
Available on backorder
Sample type
Serum, Hep Plasma, Cit plasma
Assay type
Competitive
Sensitivity
= 0.05 ng/ml
Recovery
98.66 %
Assay duration
Multiple steps standard assay
Product overview
Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.
Platform
Microplate
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