Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)- 96 Tests

Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture media, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 0.32 ng/ml
Range
0.391 ng/ml – 25 ng/ml
Recovery
103 %

Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Human alpha 1 Antitrypsin ELISA kit (SERPINA1) (ab108799) is a sandwich ELISA kit for the quantitative measurement of alpha 1 Antitrypsin in urine, milk, saliva, CSF, cell lysate and cell culture supernatants.

An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.

Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate