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Human alpha 2 Macroglobulin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.95 µg/ml
Range: 1.25 µg/ml – 40 µg/ml
Sample type: Plasma, Serum
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Human

EGP 19,152.00

Available on backorder

Sample type
Serum, Plasma
Assay type
Competitive
Sensitivity
= 0.95 µg/ml
Range
1.25 µg/ml – 40 µg/ml
Recovery
98 %

Assay time
3h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s alpha 2 Macroglobulin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in plasma and serum.

An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 2 Macroglobulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 2 Macroglobulin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108888-1x 96 test Category: Tag:

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