Human alpha Fetoprotein ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 2 ng/ml
Range: 2 ng/ml – 300 ng/ml
Sample type: Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,488.00
Available on backorder
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich (quantitative)
Sensitivity
= 2 ng/ml
Range
2 ng/ml – 300 ng/ml
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s alpha Fetoprotein (AFP) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in Human serum.
A 96-well plate has been precoated with anti-alpha Fetoprotein antibodies. Samples and standards and are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-alpha Fetoprotein-HRP conjugate binds to the antibody-alpha Fetoprotein complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.
Platform
Microplate
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