Human Apolipoprotein B ELISA Kit (APOB)- 96 Tests

Detection method
Colorimetric
Precision
Intra-assay
Sample n Mean SD CV%
Overall 5.3%
Inter-assay
Sample n Mean SD CV%
Overall 9.9%
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Cell Lysate
Assay type
Sandwich (quantitative)
Sensitivity
= 3.91 ng/ml
Range
3.91 ng/ml – 250 ng/ml
Recovery
97 %

Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Apolipoprotein B (APOB) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein B concentrations in Human plasma, serum, CSF and cell culture samples.

An Apolipoprotein B specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein B specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein B captured in plate.

Get results in 90 minutes with Human Apolipoprotein B ELISA Kit (ab190806) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate