Human Apolipoprotein E ELISA Kit (APOE)- 96 Tests

Sample type
Cell culture supernatant, Serum, Plasma, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 6.3 ng/ml
Range
0.016 µg/ml – 1 µg/ml
Recovery
97 %

Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Does not react with: Cynomolgus monkey
Product overview
Human Apolipoprotein E ELISA kit (ApoE) is designed for the quantitative measurement of Apolipoprotein E concentrations in human plasma, serum, cerebrospinal fluid and cell culture supernatant samples.

An Apolipoprotein E specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein E specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein E captured in plate. The kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate