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Human DKK1 ELISA Kit (Dickkopf-1)- 96 Tests

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Key features and details
Sensitivity: 100 pg/ml
Range: 122.9 pg/ml – 30000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,488.00

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Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 100 pg/ml Range 122.9 pg/ml - 30000 pg/ml Recovery 90 % Sample specific recovery Sample type Average % Range Cell culture supernatant 108.3 93% - 123% Serum 92.3 62% - 130% Plasma 72.8 59% - 110% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s DKK1 (Dickkopf-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimization may be required with urine samples. Platform Microplate

SKU: ab100501-1x 96 test Category: Tag:

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