Human DKK4 ELISA Kit (Dickkopf-4)- 96 Tests

Product name
Human DKK4 ELISA Kit (Dickkopf-4)
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml Range 13.72 pg/ml - 10000 pg/ml Recovery 93 % Sample specific recovery Sample type Average % Range Cell culture supernatant 109.6 97% - 121% Serum 95.59 85% - 105% Plasma 82.62 69% - 91% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s DKK4 (Dickkopf-4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK4 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human DKK4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimisation may be required with urine samples. Platform Microplate