Human Eotaxin 2 ELISA Kit (CCL24)- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 2 pg/ml Range 1.37 pg/ml - 1000 pg/ml Recovery 95 % Sample specific recovery Sample type Average % Range Cell culture supernatant 95.77 82% - 103% Serum 94.86 83% - 103% Plasma 96.74 85% - 104% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s Eotaxin 2 (CCL24) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Eotaxin 2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Eotaxin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get results in 90 minutes with Human Eotaxin-2 ELISA Kit (CCL24) (ab179882) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples. Platform Microplate