Human Fas Ligand ELISA Kit (APTL)- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 2 pg/ml Range 1.37 pg/ml - 1000 pg/ml Recovery 75 % Sample specific recovery Sample type Average % Range Cell culture supernatant 76.99 70% - 90% Serum 76.36 70% - 87% Plasma 73.42 67% - 86% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimization may be required with urine samples Platform Microplate