Human Fetuin A ELISA Kit (AHSG)- 96 Tests

Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
> 3 ng/ml
Range
3.13 ng/ml – 200 ng/ml
Recovery
= 99 %

Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Fetuin A (AHSG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A concentrations in Human plasma, serum, urine, milk and cell culture supernatants.

A Fetuin A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fetuin A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fetuin A captured in plate.

Get higher sensitivity in only 90 minutes with Human Fetuin A ELISA Kit (AHSG) (ab185989) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate