Human Galectin 7 ELISA Kit (GAL7)- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 20 pg/ml Range 13.72 pg/ml - 10000 pg/ml Recovery > 90 %

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 96.55 87% – 105%
Serum 107.1 95% – 115%
Plasma 91.82 70% – 125%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Galectin 7 (GAL7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 7 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Galectin 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Notes
Optimization may be required with urine samples

Platform
Microplate