Human IL-1 alpha ELISA Kit- 96 Tests

Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
> 0.95 pg/ml
Range
0.977 pg/ml – 250 pg/ml
Recovery
98 %

Assay time
5h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s IL-1 alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 alpha concentrations in cell culture supernatants, serum and plasma.

An IL-1 alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 alpha specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 alpha captured in plate.

Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate