Human IL-1 R4 ELISA Kit (ST2)- 96 Tests

Product name
Human IL-1 R4 ELISA Kit (ST2)
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 2 pg/ml Range 1.65 pg/ml - 1200 pg/ml Recovery 97 % Sample specific recovery Sample type Average % Range Cell culture supernatant 98.43 91% - 105% Serum 109.6 98% - 117% Plasma 84.06 67% - 102% Assay time 5h 00m Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Human IL-1 R4 ELISA Kit (ST2) (ab100563) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 R4 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human IL-1 R4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 R4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 R4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimization may be required with urine samples. Platform Microplate