Human IL-13 ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 0.15 pg/ml Range 0.16 pg/ml - 40 pg/ml Recovery 100 % Sample specific recovery Sample type Average % Range Cell culture supernatant 100.65 88% - 107% Serum 96.35 84% - 103% Plasma 94.39 82% - 102% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s IL-13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-13 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get results in 90 minutes with Human IL-13 ELISA Kit (ab178014) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples Platform Microplate