Human Interferon gamma ELISA Kit (IFNG)- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Sandwich (quantitative)
Sensitivity
< 15 pg/ml Range 20.6 pg/ml - 15000 pg/ml Recovery 92 % Sample specific recovery Sample type Average % Range Cell culture extracts 92.25 82% - 103% Tissue Extracts 91.73 80% - 101% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma in cell lysates and tissue lysates. This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range. Notes Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances. It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. Platform Microplate