Human MCP1 ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 2 pg/ml Range 2 pg/ml - 500 pg/ml Recovery 93 % Sample specific recovery Sample type Average % Range Cell culture supernatant 97.62 85% - 104% Serum 92.65 80% - 102% Plasma 90.42 79% - 101% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Predicted to work with: Mouse, Rat Product overview Abcam’s MCP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get higher sensitivity in only 90 minutes with Human MCP1 ELISA Kit (CCL2) (ab179886) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples. Platform Microplate