Human MIF ELISA Kit- 96 Tests

Product name
Human MIF ELISA Kit
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 6 pg/ml Range 8.23 pg/ml - 6000 pg/ml Recovery 94 % Sample specific recovery Sample type Average % Range Cell culture supernatant 94.68 83% - 103% Serum 93.48 82% - 102% Plasma 95.41 84% - 103% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s MIF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIF in serum, plasma (collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernatants. This assay employs an antibody specific for Human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm Notes Optimization may be required with urine samples. Platform Microplate