Human MMP10 ELISA Kit- 96 Tests

Product name
Human MMP10 ELISA Kit
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 3 pg/ml Range 2.06 pg/ml - 1500 pg/ml Recovery 96 % Sample specific recovery Sample type Average % Range Cell culture supernatant 98.31 87% - 109% Serum 96.54 85% - 108% Plasma 94.57 84% - 105% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s MMP10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP10 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernates. This assay employs an antibody specific for Human MMP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimisation may be required with urine samples. Platform Microplate