Human MMP2 ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 3.5 ng/ml Range 3.3 ng/ml - 800 ng/ml Recovery 81 % Sample specific recovery Sample type Average % Range Cell culture supernatant 77.25 69% - 92% Serum 81.27 70% - 93% Plasma 84.53 72% - 95% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s Human MMP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP2 in serum, plasma, and cell culture supernates. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay due to their chelating properties). This assay employs an antibody specific for Human MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimisation may be required with urine samples. Platform Microplate