Human PDGF AA ELISA Kit- 96 Tests

Product name
Human PDGF AA ELISA Kit
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 40 pg/ml Range 40 pg/ml - 30000 pg/ml Recovery Sample specific recovery Sample type Average % Range Cell culture supernatant 92.54 82% - 102% Serum 76.71 70% - 83% Plasma 78.06 72% - 85% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s PDGF AA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AA in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human PDGF AA coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimization may be required with urine samples Platform Microplate