Human PDGF BB ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 1 pg/ml Range 0.549 pg/ml - 400 pg/ml Recovery 93 % Sample specific recovery Sample type Average % Range Cell culture supernatant 95.43 84% - 104% Serum 93.57 82% - 103% Plasma 91.99 83% - 104% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF BB in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get results in 90 minutes with Human PDGF BB ELISA Kit (ab184860) from our SimpleStep ELISA® range. Notes Note: Optimization may be required with urine samples, and very low level are detectable in serum Platform Microplate