Human Plasminogen ELISA Kit (PLG)- 96 Tests

Sample type
Cell culture supernatant, Urine, Serum, Plasma, Tissue
Assay type
Sandwich (quantitative)
Sensitivity
= 0.3 ng/ml
Range
1.25 ng/ml – 80 ng/ml
Recovery
= 101 %

Assay time
3h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Human Plasminogen ELISA kit (PLG) is designed for the quantitative measurement of Plasminogen (PLG) concentrations in Human plasma, serum, urine, saliva, milk, tissue samples, cerebrospinal fluid and cell culture supernatants.

A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.

Get higher sensitivity in only 90 minutes with Human Plasminogen ELISA Kit (ab196262) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate