Human RAGE ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 3 pg/ml Range 2.06 pg/ml - 1500 pg/ml Recovery 90 % Sample specific recovery Sample type Average % Range Cell culture supernatant 103.4 94% - 112% Serum 89.25 79% - 98% Plasma 78.58 67% - 93% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s RAGE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RAGE in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get higher sensitivity in only 90 minutes with Human RAGE ELISA Kit (ab190807) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples. Platform Microplate