Human SDF1 alpha ELISA Kit- 96 Tests

Detection method
Colorimetric
Precision
Intra-assay
Sample n Mean SD CV%
Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% Sample type Cell culture supernatant, Plasma Assay type Sandwich (quantitative) Sensitivity < 80 pg/ml Range 93.8 pg/ml - 6000 pg/ml Recovery > 80 %

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 94.63 86% – 104%
Plasma 89.34 85% – 104%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Human SDF1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100637) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SDF1 alpha in plasma and cell culture supernatants. (Human SDF1 alpha concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human SDF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Human SDF-1 alpha in normal serum with ab100637, only in serum spiked with Human SDF-1 alpha.

Notes
Optimization may be required with urine samples.

Platform
Microplate