Human Siglec 9 ELISA Kit (CD329)- 96 Tests

Product name
Human Siglec 9 ELISA Kit (CD329)
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 130 pg/ml Range 0.123 ng/ml - 30 ng/ml Recovery 85 % Sample specific recovery Sample type Average % Range Cell culture supernatant 95.43 88% - 102% Serum 82.7 72% - 91% Plasma 78.16 70% - 91% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s Siglec 9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Siglec 9 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for Human Siglec 9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Siglec 9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimization may be required with urine samples. Platform Microplate