Human VEGF ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml Range 8.23 pg/ml - 6000 pg/ml Recovery 96 % Sample specific recovery Sample type Average % Range Cell culture extracts 96.79 90% - 107% Tissue Extracts 95.58 89% - 106% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in cell lysates and tissue lysates. This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances. It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. Platform Microplate